aeruginosa PBS in minimal salt medium (pH 7), possessing 2.17% sodium citrate as C-source and 0.5% yeast extract as N-source, after 48 h upon incubation at 30 ☌/150 rpm.
The maximum biosurfactant yield was attained with 2% fresh inoculum of P. The optimization of media components and process parameters was consecutively executed in two sets of experimental runs designed by central composite rotatable design (CCRD). The biosurfactant production medium and culture conditions were optimized using response surface methodology. aeruginosa PBS for microbial enhanced oil recovery (MEOR).
The present work reveals the potential of biosurfactant producing P.
